Date of Award
7-1974
Degree Type
Thesis
Degree Name
Master of Science (MS)
Department
Biology
Supervisor
Professor S. T. Bayley
Abstract
This assignment of codons in Halobacterium cutirubrum for some of the acidic and basic amino acids was undertaken. Although trinucleotides could not be used successfully in the halophile ribosomal binding system, the unfractionated aminoacyl-tRNAs responded to the established codons in the heterologous binding system using E. coli ribosomes. This suggested that H. cutirubrum uses the established code in full.
Two species of lysine accepting tRNA were purified. Their coding response to Poly(A) and Poly(A,G) was studied. tRNA[Lys,1] recognized both AAA and AAG codons while tRNA[Lys,2] recognized AAA preferentially in both the homologous and heterologous ribosomal binding systems.
Recommended Citation
Patel, Jayantkumar, "Coding Properties of Some tRNAs from Halobacterium cutirubrum" (1974). Open Access Dissertations and Theses. Paper 530.
http://digitalcommons.mcmaster.ca/opendissertations/530