Date of Award

Spring 2012

Degree Type


Degree Name

Master of Applied Science (MASc)


Electrical and Computer Engineering


Thomas E. Doyle


Matiar Howlader




An electro-optical sensor module was designed to monitor the level of dissolved oxygen (DO) using the method of frequency domain fluoroscopy. Frequency domain fluoroscopy is an optical method that detects the concentration of an analyte by indirectly monitoring the fluorescent lifetime decay. A planar film containing oxygen sensitive fluorophores interacts with a liquid solution, where the percent DO dictates the fluorescent lifetime decay. Amplitude modulated LED emission is created using an electrically implemented oscillator, exciting the oxygen sensitive fluorophores. The emission light from the fluorophores is detected by a photodiode and conditioned. The timing characteristics of the excitation and emission light waveforms are interpreted by a microcontroller. Time delay values have been correlated to actual percent DO values experimentally, and appropriate data modeling has been implemented for calibration purposes. This design is appropriate for application in bioreactors, presenting a functional and cost effective design. Future research can be performed to extrapolate the microcontroller platform to host a pH module, cell number module and glucose module, providing sufficient feedback to an automated bioreactor systems.

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